A NOVEL METHOD OF PURIFICATION OF SPECIFIC HYDATID CYST ANTIGEN
Authors
Abstract:
Hydatid cyst fluid has been used as a source of antigen for the serodiagnosis of Echinococcus granulosus infection. Due to cross-reactions with antigens shared by other helminthes, the specific antigen from hydatid cyst fluid was purified by many workers. We used a relatively simple technique for purification of specific antigen from sheep hydatid cyst fluid. Isopycnic ultracentrifugation with 30% KEr was used followed by SDS-PAGE to check the purity of the antigen. The antigen was of 48 kDa and used in ELISA and IRA with high sensitivity and specificity.
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a novel method of purification of specific hydatid cyst antigen
hydatid cyst fluid has been used as a source of antigen for the serodiagnosis of echinococcus granulosus infection. due to cross-reactions with antigens shared by other helminthes, the specific antigen from hydatid cyst fluid was purified by many workers. we used a relatively simple technique for purification of specific antigen from sheep hydatid cyst fluid. isopycnic ultracentrifugation with ...
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full textidentification and purification of a specific and immunogenic antigen of the laminated layer of the hydatid cyst and production of an antigen-specific monoclonal antibody.
cystic echinococcosis (ce) is an infection caused by the larval stage of echinococcus granulosus. this is widely distributed through iran, where a variety of animals act as intermediate host. the immunogenic antigens (ag) of different compartments of the hydatid cyst have been already determined. one of these compartments is the laminated layer (ll). we have extracted a protein with the mw of 2...
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Journal title
volume 15 issue 1
pages 49- 53
publication date 2001-05
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